Investigation of the Best Saccharomyces cerevisiae Growth Condition

Authors

  • Rosita Salari Ph.D. of Food Science and Technology, Food and Drug Administration, Mashhad University of Medical Sciences, Mashhad, Iran

Keywords:

Carrier, Growth, Mathematics, Saccharomyces cerevisiae yeast cells

Abstract

Introduction: Saccharomyces cerevisiae is known as one of the useful yeasts which are utilized in baking and other industries.  It can be easily cultured at an economic price. Today the introduction of safe and efficient carriers is being considered. Due to its generally round shape, and the volume that is enclosed by its membrane and cell wall, it is used to encapsulate active materials to protect them from degradation or to introduce a sustained release drug delivery system. Providing the best conditions in order to achieve the best morphological properties of Saccharomyces cerevisiae as a carrier.

Methods: In this research, the most suitable growth condition of yeast cells which provides the best size for use as drug carriers was found by a bioreactor in a synthetic culture medium. Yeast cell reproduction and growth curves were obtained, based on pour plate colony counting data and UV/Visible sample absorption at 600 nm. Yeast cell growth patterns and growth rates were determined by Matlab mathematical software.

Results: Results showed that pH=4 and dissolving oxygen (DO) 5% was the best condition for yeast cells to grow and reproduce. This condition also provided the largest size (2 × 3 µ) yeast cells. 

Conclusion: Owing to the yeast cells’ low-cost production and their structural characteristics, they could be used as potent drug carriers.

Funding: This work was supported by a grant from the Vice Chancellor of Research of Mashhad University of Medical Sciences.

References

Feldmann H. Yeast: Molecular and Cell Biology. Wiley-Blackwell. 2010.

Walker LJ, Aldhous MC, Drummond HE, Smith BR, Nimmo ER, Arnott ID, et al. Anti-Saccharomyces

cerevisiae antibodies (ASCA) in Crohn's disease are associated with disease severity but not

NOD2/CARD15 mutations. Clin Exp Immunol. 2004; 135(3): 490-6. doi: 10.1111/j.1365- 2249.2003.02392.x. PMID: 15008984, PMCID: PMC1808965.

Friedman N. The Friedman Lab Chronicles. Growing yeasts (Robotically). Nir Friedman Lab. 2011.

Herskowitz I. Life cycle of the budding yeast Saccharomyces cerevisiae. Microbiol Rev. 1988; 52(4): 536- 53. PMID: 3070323, PMCID: PMC373162.

Kaeberlein M, Powers RW, Steffen KK, Westman EA, Hu D, Dang N, et al. Regulation of yeast replicative

life span by TOR and Sch9 in response to nutrients. Science. 2005; 310(5751): 1193-6. doi:

1126/science.1115535. PMID: 16293764.

Kaeberlein M. Lessons on longevity from budding yeast. Nature. 2010; 464(7288): 513-9. doi:

1038/nature08981. PMID: 20336133, PMCID: PMC3696189.

Kraft P, Pharoah P, Chanock SJ, Albanes D, Kolonel LN, Hayes RB, et al. Genetic variation in the

HSD17B1 gene and risk of prostate cancer. PLoS Genet. 2005; 1(5): 68. PMID: 16311626, PMCID:

PMC1287955.

De Nobel JG, Klis FM, Munnik T, Priem J, Van Den Ende H. An assay of the relative cell wall porosity of

Saccharomyces cerevisiae, Kluveromyces lactis and Schizosaccharomyces pombe. Yeast. 1990; 6(6): 483- 90. doi: 10.1002/yea.320060605. PMID: 2080665.

Sadeghi F, Torab M, Khattab M, Homayouni A, Afrasiabi Garekani H. Improvement of Physico- mechanical Properties of Partially Amorphous Acetaminophen Developed from Hydroalcoholic Solution

Using Spray Drying Technique. Iran J Basic Med Sci. 2013; 16(10): 1100-8. PMID: 24379968, PMCID:

PMC3874097.

Shi G, Rao L, Yu H, Xiang H, Pen G, Long S, et al. Yeast-cell based microencapsulation of chlorogenic

acid as a water-soluble antioxidant. J Food Eng. 2007; 80(4): 1060-7. doi: 10.1016/j.jfoodeng.2006.06.038.

Buis R. On the Generalization of the Logistic Law of Growth. Acta Biotheoretica. 1991; 39(3): 185-95.

doi: 10.1007/BF00114174.

Marchetti C, Nakicenovic N. The Dynamics of Energy Systems and the Logistic Substitution Model. Int

Inst For Appl Sys. Laxenburg, Austria. 1980.

Nelder JA. The fitting of a generalization of the logistic curve. Biometrics. 1961; 17: 89-110. doi:

2307/2527498.

Tsoularis A, Wallace J. Analysis of logistic growth models. Math Biosci. 2002; 179(1): 21-55. PMID:

Turner ME Jr, Blumenstein BA, Sebaugh JL. A generalization of the logistic law of growth. Biometrics.

; 25(3): 577-80. doi: 10.2307/2528910. PMID: 5824407.

Richards OW. The Growth of the Yeast Saccharomyces cerevisiae. Ann Bot. 1928; 42(1): 271-83.

Serrano R, Martín H, Casamayor A, Ariño J. Signaling alkaline pH stress in the yeast Saccharomyces

cerevisiae through the Wsc1 cell surface sensor and the Slt2 MAPK pathway. J Biol Chem. 2006; 281(52):

-95. doi: 10.1074/jbc.M604497200. PMID: 17088254.

Salari R, Fazly Bazzaz BS, Rajabi O, Khashyarmanesh Z. New aspects of Saccharomyces cerevisiae as a

novel carrier for berberine. DARU 2013; 21:73. DOI: 10.1186/2008-2231-21-73.

Salari R, Rajabi O, Khashyarmanesh Z, Fathi Najafi M, Fazly Bazzaz BS. Characterization of Encapsulated

Berberine in Yeast Cells of Saccharomyces cerevisiae. Iran J Pharm Res 2015; 14(4): 1247–1256. PMid:

, PMCid: PMC4673954

Published

2022-01-18