Effect of non-ionizing electromagnetic field on the alteration of ovarian follicles in rats

Authors

  • Amir Afshin Khaki Ph.D. of Anatomy, Associate Professor, Tabriz Health Services Management Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Keywords:

electromagnetic field, follicle, rats, infertility

Abstract

Introduction: In recent years, there has been an increase in the attention paid to safety effects, environmental and society’s health, extremely low frequency electromagnetic fields (ELF-EMF), and radio frequency electromagnetic fields (RF-EMF). The aim of this research was to determine the effect of EMF on the alteration of ovarian follicles. 

Methods: In this experimental study at Tabriz Medical University in 2015, we did EMF exposures and assessed the alteration of rats’ ovarian follicles. Thirty three-month old rats were selected randomly from laboratory animals, and, after their ages and weights were determined, they were divided randomly into three groups. The control group consisted of 10 rats without any treatment, and they were kept in normal conditions. The second group of rats was influenced by a magnetic field of 50 Hz for eight weeks (three weeks intrauterine and five weeks ectopic). The third group of rats was influenced by a magnetic field of 50 Hz for 13 weeks (three weeks intrauterine and ten weeks ectopic). Samples were fixed in 10% buffered formaldehyde and cleared with Xylol and embedded in paraffin. After sectioning and staining, samples were studied by optic microscopy. Finally, SPSS version 17, were used for data analysis.  

Results: EMF radiation increased the harmful effects on the formation of ovarian follicles and oocytes implantation. Studies on the effects of electromagnetic fields on ovarian follicles have shown that the nuclei of the oocytes become smaller and change shape. There were significant, harmful changes in the groups affected by electromagnetic waves. Atresia of ovarian follicles was significantly significant in both study groups compared to the control group (p < 0.05). 

Conclusion: Exposure to electromagnetic fields during embryonic development can cause morphological changes in oocytes and affect the differentiation of oocytes and folliculogenesis, resulting in decreased ovarian reserve leading to infertility or reduced fertility.

References

D’Angelo C, Costantini E, Kamal M, Reale M. Experimental model for ELF-EMF exposure: Concern for

human health. Saudi J Biol Sci. 2015; 22(1): 75-84. doi: 10.1016/j.sjbs.2014.07.006, PMID: 25561888,

PMCID: PMC4281612.

Hajhosseini L, Khaki A, Merat E, Ainehchi N. Effect of rosmarinic acid on sertoli cells apoptosis and

serum antioxidant levels in rats after exposure to electromagnetic fields. Afr J Tradit Complement Altern

Med. 2013; 10(6): 477-80. PMCID: PMC3847387.

Ujházy E, Mach M, Navarová J, Brucknerová I, Dubovický M. Teratology–past, present and future.

Interdiscip Toxicol. 2012; 5(4): 163-8. doi: 10.2478/v10102-012-0027-0, PMID: 23554558.

Khaki AA, Zarrintan S, Khaki A, Zahedi A. The effects of electromagnetic field on the microstructure of

seminal vesicles in rat: a light and transmission electron microscope study. Pak J Biol Sci. 2008; 11(5):

-701. doi: 10.3923/pjbs.2008.692.701, PMID: 18819564.

Khaki AA, Tubbs R, Shoja M, Rad J, Khaki A, Farahani R, et al. The effects of an electromagnetic field on

the boundary tissue of the seminiferous tubules of the rat: A light and transmission electron microscope

study. Folia Morphol. 2006; 65(3): 188-94. PMID: 16988914.

Roshangar L, Hamdi B, Khaki A, Rad JS, Soleimani-Rad S. Effect of low-frequency electromagnetic field

exposure on oocyte differentiation and follicular development. Adv biomed Res. 2014; 3: 76. doi:

4103/2277-9175.125874, PMID: 24627884, PMCID: PMC3950798.

Cecconi S, Gualtieri G, Di Bartolomeo A, Troiani G, Cifone MG, Canipari R. Evaluation of the effects of

extremely low frequency electromagnetic fields on mammalian follicle development. Hum Reprod. 2000;

(11): 2319-25. doi: 10.1093/humrep/15.11.2319, PMID: 11056125.

Khaki AA, Gharachurlou K, Rad J, Tajik P, Sohrabi I, Khaki A, et al. editors. The effects of

electromagnetic field (EMF) on ovary in rat (a light microscopic study). PLACENTA; 2008: WB

SAUNDERS CO LTD 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND.

Rajaei F, Borhani N, Sabbagh-Ziarani F, Mashayekhi F. Effects of extremely low-frequency

electromagnetic field on fertility and heights of epithelial cells in pre-implantation stage endometrium and

fallopian tube in mice. Zhong Xi Yi Jie He Xue Bao. 2010; 8(1): 56. doi: 10.3736/jcim20100111, PMID:

Cao Y, Zhang Y, Liu Y. [Effects of exposure to extremely low frequency electromagnetic fields on

reproduction of female mice and development of offsprings]. Zhonghua lao dong wei sheng zhi ye bing za

zhi. 2006; 24(8): 468-70. PMID: 16978513.

de Vocht F, Hannam K, Baker P, Agius R. Maternal residential proximity to sources of extremely low

frequency electromagnetic fields and adverse birth outcomes in a UK cohort. Bioelectromagnetics. 2014;

(3): 201-9. doi: 10.1002/bem.21840.

Wu YL, Ma SR, Peng T, Teng ZH, Liang XY, Guo GZ, et al. Effects of Pulsed Electromagnetic Field on

Differentiation of HUES-17 Human Embryonic Stem Cell Line. Int J Mol Sci. 2014; 15(8): 14180-90. doi:

3390/ijms150814180, PMID: 25196518, PMCID: PMC4159845.

Hug K, Grize L, Seidler A, Kaatsch P, Schüz J. Parental occupational exposure to extremely low frequency

magnetic fields and childhood cancer: a German case-control study. Am J Epidemiol. 2010; 171(1): 27-35.

doi: 10.1093/aje/kwp339, PMID: 19942577.

Bakacak M, Bostancı MS, Attar R, Yıldırım ÖK, Yıldırım G, Bakacak Z, et al. The effects of

electromagnetic fields on the number of ovarian primordial follicles: An experimental study. Kaohsiung J

Med Sci. 2015; 31(6): 287-92. doi: 10.1016/j.kjms.2015.03.004, PMID: 26043407.

Mevissen M, Buntenkötter S, Löscher W. Effects of static and time‐varying (50‐Hz) magnetic fields on

reproduction and fetal development in rats. Teratology. 1994; 50(3): 229-37. doi:

1002/tera.1420500308, PMID: 7871487.

Chung MK, Kim JC, Myung SH, Lee DI. Developmental toxicity evaluation of ELF magnetic fields in

Sprague–Dawley rats. Bioelectromagnetics. 2003; 24(4): 231-40. doi: 10.1002/bem.10114, PMID:

Chung MK, Kim JC, Myung SH. Lack of adverse effects in pregnant/lactating female rats and their

offspring following pre-and postnatal exposure to ELF magnetic fields. Bioelectromagnetics. 2004; 25(4):

-44. doi: 10.1002/bem.10182, PMID: 15114632.

Portier CJ, Wolfe MS. Assessment of health effects from exposure to power-line frequency electric and

magnetic fields. NIH publication. 1998; 98: 3981.

Huuskonen H, Juutilainen J, Komulainen H. Effects of low ‐ frequency magnetic fields on fetal

development in rats. Bioelectromagnetics. 1993; 14(3): 205-13. doi: 10.1002/bem.2250140304, PMID:

Da Silva-Buttkus P, Jayasooriya GS, Mora JM, Mobberley M, Ryder TA, Baithun M, et al. Effect of cell

shape and packing density on granulosa cell proliferation and formation of multiple layers during early

follicle development in the ovary. J Cell Sci. 2008; 121(23): 3890-900. doi: 10.1242/jcs.036400.

Khaki A, Khaki AA, Zahedi A. Effect of Ocimum basilicum on Ovary tissue Apoptosis after exposed with

extremely low frequency electromagnetic fields (ELF-EMF) in Rats. Life Sci J. 2013; 10(3s).

Klinger FG, De Felici M. In vitro development of growing oocytes from fetal mouse oocytes: stage-specific

regulation by stem cell factor and granulosa cells. Dev Biol. 2002; 244(1): 85-95. doi:

1006/dbio.2002.0592, PMID: 11900461.

Roushangar L, Rad JS. Ultrastructural alterations and occurrence of apoptosis in developing follicles

exposed to low frequency electromagnetic field in rat ovary. Pak J Biol Sci. 2007; 10(24): 4413. doi:

3923/pjbs.2007.4413.4419, PMID: 19093504.

Khaki A, Khaki AA. Recovery Effect of Basil on ovarian tissue artery Hyperemia after exposed with

electromagnetic field. Health Med. 2013; 7(8): 2375.

Published

2022-03-07

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