Effect of Selenium on Neurotoxicity in Adult Male Mice Exposed to Formaldehyde
Keywords:
formaldehyde, cerebellum, morphometric, mice, neurotoxicity, seleniumAbstract
Background: Formaldehyde is used in medicine and industry, and it is known to have detrimental effects on various systems including the nervous system, by increasing oxidative stress. However, data are scarce related to substances that can protect against the neurotoxicity induced by formaldehyde. Therefore, this study was designed to assess the protective effects of selenium against the toxic effect of this compound.
Methods: A total of 48 adult male mice were divided randomly into six groups, i.e., (1) control, (2) treated with formaldehyde, (3) treated with formaldehyde plus 0.1 mg/kg selenium, (4) treated with formaldehyde plus 0.2 mg/kg selenium, (5) treated with formaldehyde plus 0.4 mg/kg selenium, and (6) treated with formaldehyde plus 0.8 mg/kg selenium. At the end of 14 days, the cerebellums were removed for histological evaluation. Morphological changes were examined using Image J software. The data were analyzed using SPSS software version 20.0 and analysis of variance (ANOVA).
Results: Formaldehyde caused a reduction in the numbers and sizes of Purkinje cells and granular cells; in addition, the thickness of the granular layer was thinner than that in the control mice (P < 0.05). Treatment with 0.1 mg/kg selenium resulted in an increase in the number of Purkinje cells as well as the area of the gray matter compared to those of the control mice.
Conclusion: Formaldehyde-induced neuronal damage was prevented by the administration of 0.1 mg/kg selenium, hence this treatment shows therapeutic potential for the treatment of neurotoxicity.
References
Usanmaz SE, Akarsu ES, Vural N. Neurotoxic effects of acute and subacute formaldehyde exposures inmice. Envir Toxicol Pharmacol 2002: 11: 93-100.http://dx.doi.org/10.1016/S1382-6689(01)00109-02.Mehmet İnci, İsmail Zararsız, Mürsel Davarcı, Sadık Görür. Toxic effects of formaldehyde on the urinarysystem. Turkish Journal of Urology 2013; 39(1): 48-52.http://dx.doi.org/10.5152/tud.2013.0103.Wong EY, Ray R, Gao DL, Wernli KJ, Li W, Fitzgibbons ED, et al. Reproductive history, occupationalexposures, and thyroid cancer risk among women textile workers in Shanghai, China. Int Arch OccupEnviron Health 2006; 79: 251–8.http://dx.doi.org/10.1007/s00420-005-0036-9. PMid:162202874.Yu LQ, Jiang SF, Leng SG,He FS, Zheng YX. Early genetic effects on workers occupationally exposed toformaldehyde. Zhonghua Yu Fang Yi Xue Za Zhi 2005; 39: 392–5. PMid:163297985.Cheng G, Shi Y, Sturla SJ, Jalas JR, McIntee EJ, Villalta PW, et al. Reactions of formaldehyde plusacetaldehyde with deoxyguanosine and DNA: formation of cyclic deoxyguanosine adducts andformaldehyde cross-links. Chem Res Toxicol 2003; 16: 145–52.http://dx.doi.org/10.1021/tx025614r.PMid:125881856.Metz B, Kersten GF, Hoogerhout P, Brugghe HF, Timmermans HA, de Jong A, et al. Identification offormaldehyde-induced modifications in proteins: reactions with model peptides. J Biol Chem 2004; 279:6235–43.http://dx.doi.org/10.1074/jbc.M310752200 PMid:146386857.Kriebel D, Myers D, Cheng M, Woskie S, Cocanour B. Short term effect of formaldehyde on peakexpiratory flow and irritant symptoms. Arch Environ Health 2001; 56:11–18.http://dx.doi.org/10.1080/000398901096040498.Sarsilmaz M, Kaplan S, Songur A, Colakoglu S, Aslan H, Tunc AT, Ozen OA, Turgut M, Bas O. Effects ofpostnatal formaldehyde exposure on pyramidal cell number, volumeof cell layer in hippocampus andhemisphere in the rat: a stereological study. Brain Res 2007;1145:157–167.http://dx.doi.org/10.1016/j.brainres.2007.01.139.PMid:173466819.Ozen OA, Akpolat N, Songur A, Kuş I, Zararsiz I, Ozaçmak VH, et al. Effect of formaldehyde inhalationon Hsp70 in seminiferous tubules of rat testes: an immunohistochemical study. Toxicol Ind Health2005;21:249–54.http://dx.doi.org/10.1191/0748233705th235oaPMid:1646395710.Gurel A, Coskun O, Armutcu F, Kanter M, Ozen OA. Vitamin E against oxidative damage caused byformaldehyde in frontal cortex and hippocampus: biochemical and histological studies. J Chem Neuroanat2005; 29: 173–8.http://dx.doi.org/10.1016/j.jchemneu.2005.01.001.PMid:15820619
Saito Y, Nishio K, Yoshida Y, Niki E. Cytotoxic effect of formaldehyde with free radicals via increment ofcellular reactive oxygen species. Toxicology 2005; 210: 235–45.http://dx.doi.org/10.1016/j.tox.2005.02.006.PMid:1584043712.Mohammadi S, Movahedin M, Mowla SJ. Up-regulation of CatSper genes family by selenium. ReprodBiol Endocrinol 2009; 7: 126, 1-6. doi: 10.1186/1477-7827-7-126. PMID:1991709813.Ismail Zararsiz, Ilter Kus, Murat Ogeturk, Nusret Akpolat, Evren Kose, Sedat Meydan , MustafaSarsilmaz. Melatonin prevents formaldehyde-induced neurotoxicity in prefrontal cortex of rats: animmunohistochemical and biochemical study. Cell Biochem Funct 2007; 25: 413–418.http://dx.doi.org/10.1002/cbf.1315.PMid:1639790514.Kabuto H, Yokoi I, Ogawa N. Melatonin inhibits iron-induced epileptic discharges in rats by suppressingperoxidation. Epilepsia 1998; 39: 237–243.http://dx.doi.org/10.1111/j.1528-1157.1998.tb01367.xPMid:957803915.Ahmet Gurel, Omer Coskun, Ferah Armutcu, Mehmet Kanter, Oguz Aslan Ozen. Vitamin E againstoxidative damage caused by formaldehyde in frontal cortex and hippocampus: Biochemical andhistological studies. Journal of Chemical Neuroanatomy 29 .2005; 173–178. DOI:10.1016/j.jchemneu.2005.01.001 PMID:15820619.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Knowledge Kingdom Publishing
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.