NF-E2-related factor 2 over-expression in mesenchymal stem cells to improve cellular cardiomyoplasty
Keywords:
NF-E2-Related Factor 2 (Nrf2); Mesenchymal Stromal Cells; Myocardial Infarction; CardiomyoplastyAbstract
Myocardial infarction (MI) is the leading cause of death worldwide. Various therapeutic strategies have been introduced for MI treatment. In recent years, interest in utilizing mesenchymal stem cells (MSCs) for MI therapy has increased. In fact, the use of MSCs for MI treatment, known as cellular cardiomyoplasty, is in the clinical trial stage. However, despite promising results, most MSCs die after transplantation as a result of exposure to various stresses. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a well-known cytoprotective transcription factor, protects MSCs against some stresses. Over-expression of Nrf2 in MSCs decreases their apoptosis in vitro without any adverse effects on their differentiation capacity. Therefore, we hypothesized that over-expression of Nrf2 in MSCs can improve cellular cardiomyoplasty.
References
Mazo M, Arana M, Pelacho B, Prosper F. Mesenchymal Stem Cells and Cardiovascular Disease: A Benchto Bedside Roadmap. Stem Cells Int. 2012;2012:175979. PMID: 22315617. DOI:http://dx.doi.org/10.1155/2012/1759792.Mazo M, Pelacho B, Prosper F. Stem cell therapy for chronic myocardial infarction. J Cardiovasc TranslRes. 2010;3(2):79-88. PMID: 20560022. DOI:http://dx.doi.org/10.1007/s12265-009-9159-93.Arminan A, Gandia C, Garcia-Verdugo JM, Lledo E, Trigueros C, Ruiz-Sauri A, et al. Mesenchymal stemcells provide better results than hematopoietic precursors for the treatment of myocardial infarction. JAm Coll Cardiol. 2010;55(20):2244-53. PMID: 20466205. DOI:http://dx.doi.org/10.1016/j.jacc.2009.08.0924.BarbosaVA, Luciano TF, Vitto MF, Cesconetto PA, Marques SO, Souza DR, et al. Exercise training playscardioprotection through the oxidative stress reduction in obese rats submitted to myocardialinfarction. Int J Cardiol. 2012;157(3):422-4. PMID: 22498422. DOI:http://dx.doi.org/10.1016/j.ijcard.2012.03.1535.de Lorgeril M, Salen P, Accominotti M, Cadau M, Steghens J-P, Boucher F, et al. Dietary and bloodantioxidants in patients with chronic heart failure. Insights into the potential importance of selenium inheart failure. Eur J Heart Fail. 2001;3(6):661-9. PMID: 11738217. DOI:http://dx.doi.org/10.1016/S1388-9842(01)00179-96.Lorgis L,Zeller M, Dentan G, Sicard P, Richard C, Buffet P, et al. The free oxygen radicals test (FORT) toassess circulating oxidative stress in patients with acute myocardial infarction. Atherosclerosis.2010;213(2):616-21. PMID: 20947086. DOI:http://dx.doi.org/10.1016/j.atherosclerosis.2010.09.0187.Ceconi C, Cargnoni A, Pasini E, Condorelli E, Curello S, Ferrari R. Evaluation of phospholipidperoxidation as malondialdehyde during myocardial ischemia and reperfusion injury. Am J Physiol.91;260(4):H1057-H61. PMID: 2012211.8.Hirasawa F, Kawarada Y, Sato M, Suzuki S, Terada K, Miura N, et al. The effect of silver administrationon the biosynthesis and the molecular properties of rat ceruloplasmin. Biochim Biophys Acta.1997;1336(2):195-201. PMID: 9305790. DOI:http://dx.doi.org/10.1016/S0304-4165(97)00026-39.Berry MF, Engler AJ, Woo YJ, Pirolli TJ, Bish LT, Jayasankar V, et al. Mesenchymal stem cell injectionafter myocardial infarction improves myocardial compliance. Am J Physiol Heart Circ Physiol.2006;290(6):H2196-H203. PMID: 16473959. DOI:http://dx.doi.org/10.1152/ajpheart.01017.200510.Mohammadzadeh M, Halabian R, Gharehbaghian A, Amirizadeh N, Jahanian-Najafabadi A, RoushandehAM, et al. Nrf-2 overexpression in mesenchymal stem cells reduces oxidative stress-induced apoptosisand cytotoxicity. Cell Stress Chaperones. 2012:1-13. PMID: 22362068. DOI:http://dx.doi.org/10.1007/s12192-012-0331-911.Wang T, Sun S, Wan Z, Weil MH, Tang W. Effects of bone marrow mesenchymal stem cells in a rat modelof myocardial infarction. Resuscitation. 2012. PMID: 22450658. DOI:http://dx.doi.org/10.1016/j.resuscitation.2012.02.03312.Madonna R, Geng Y-J, De Caterina R. Adipose tissue-derived stem cells characterization and potential forcardiovascular repair. Arterioscler Thromb Vasc Biol. 2009;29(11):1723-9. PMID: 19628786. DOI:http://dx.doi.org/10.1161/ATVBAHA.109.18717913.Toma C, Pittenger MF, Cahill KS, Byrne BJ, Kessler PD. Human mesenchymal stem cells differentiate to acardiomyocyte phenotype in the adult murine heart. Circulation. 2002;105(1):93-8. PMID: 11772882.DOI:http://dx.doi.org/10.1161/hc0102.10144214.Kolossov E, Bostani T, Roell W, Breitbach M, Pillekamp F, Nygren JM, et al. Engraftment of engineeredES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarctedmyocardium. J Exp Med. 2006;203(10):2315-27. PMID:16954371. PMCID:PMC2118112. DOI:http://dx.doi.org/10.1084/jem.2006146915.Liu XB, Jiang J, Gui C, Hu XY, Xiang MX, Wang JA. Angiopoietin-1 protects mesenchymal stem cellsagainst serum deprivation and hypoxia-induced apoptosis through the PI3K/Akt pathway. ActaPharmacol Sin. 2008;29(7):815-22. PMID: 18565279. DOI:http://dx.doi.org/10.1111/j.1745-7254.2008.00811.x16.Lunde K, Solheim S, Aakhus S, Arnesen H, Abdelnoor M, Egeland T, et al. Intracoronary injection ofmononuclear bone marrow cells in acute myocardial infarction. N Engl J Med. 2006;355(12):1199-209. PMID: 16990383. DOI:http://dx.doi.org/10.1056/NEJMoa055706
Zhang M, Methot D, Poppa V, Fujio Y, Walsh K, Murry CE. Cardiomyocyte grafting for cardiac repair:graft cell death and anti-death strategies. J Mol Cell Cardiol. 2001;33(5):907-21. PMID: 11343414.DOI:http://dx.doi.org/10.1006/jmcc.2001.136718.Zhu W, Chen J, Cong X, Hu S, Chen X. Hypoxia and serum deprivation‐induced apoptosis inmesenchymal stem cells. Stem Cells. 2006;24(2):416-25. PMID: 16253984. DOI:http://dx.doi.org/10.1634/stemcells.2005-012119.Hamedi-Asl P, Halabian R, Bahmani P, Mohammadipour M, Mohammadzadeh M, Roushandeh AM, et al.Adenovirus-mediated expression of the HO-1 protein within MSCs decreased cytotoxicity andinhibited apoptosis induced by oxidative stresses. Cell Stress Chaperones. 2012;17(2):181-90. PMID:21993906. DOI:http://dx.doi.org/10.1007/s12192-011-0298-y20.Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, et al. Overexpressing cellular repressor of E1A-stimulatedgenes protects mesenchymal stem cells against hypoxia-and serum deprivation-induced apoptosis byactivation of PI3K/Akt. Apoptosis. 2010;15(4):463-73. PMID: 19997978. DOI:http://dx.doi.org/10.1007/s10495-009-0434-721.Iwase T, Nagaya N, Fujii T, Itoh T, Murakami S, Matsumoto T, et al. Comparison of angiogenic potencybetween mesenchymal stem cells and mononuclear cells in a rat model of hindlimb ischemia.Cardiovasc Res. 2005;66(3):543. PMID: 15914119. DOI:http://dx.doi.org/10.1016/j.cardiores.2005.02.00622.Li W, Kong AN. Molecular mechanisms of Nrf2‐mediated antioxidant response. Mol Carcinog.2009;48(2):91-104. PMID: 18618599, PMCID:PMC2631094,DOI:http://dx.doi.org/10.1002/mc.2046523.Lee J-M, Calkins MJ, Chan K, Kan YW, Johnson JA. Identification of the NF-E2-related factor-2-dependent genes conferring protection against oxidative stress in primary cortical astrocytes usingoligonucleotide microarray analysis. J Biol Chem. 2003;278(14):12029-38. PMID: 12556532. DOI:http://dx.doi.org/10.1074/jbc.M21155820024.Nguyen T, Nioi P, Pickett CB. The Nrf2-antioxidant response elementsignaling pathway and its activationby oxidative stress. J Biol Chem. 2009;284(20):13291-5. PMID: 19182219. PMCID:PMC2679427.DOI:http://dx.doi.org/10.1074/jbc.R90001020025.Reisman SA, Yeager RL,Yamamoto M, Klaassen CD. Increased Nrf2 activation in livers from Keap1-knockdown mice increases expression of cytoprotective genes that detoxify electrophiles more thanthose that detoxify reactive oxygen species. Toxicol Sci. 2009;108(1):35-47. PMID: 19129213.PMCID:PMC2644398. DOI:http://dx.doi.org/10.1093/toxsci/kfn26726.Homma S, Ishii Y, Morishima Y, Yamadori T, Matsuno Y, Haraguchi N, et al. Nrf2 enhances cellproliferation and resistance to anticancer drugs in human lung cancer. Clin Cancer Res.2009;15(10):3423-32. PMID: 19417020. DOI:http://dx.doi.org/10.1158/1078-0432.CCR-08-282227.Kobayashi A, Kang M-I, Okawa H, Ohtsuji M, Zenke Y, Chiba T, et al. Oxidative stress sensor Keap1functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol CellBiol. 2004;24(16):7130-9. PMID: 15282312. PMCID:PMC479737. DOI:http://dx.doi.org/10.1128/MCB.24.16.7130-7139.200428.Zhang DD, Lo S-C, Cross JV, Templeton DJ, Hannink M. Keap1 is a redox-regulated substrate adaptorprotein for a Cul3-dependent ubiquitin ligase complex. Mol Cell Biol. 2004;24(24):10941-53. PMID:15572695. PMCID:PMC533977. DOI:http://dx.doi.org/10.1128/MCB.24.24.10941-10953.200429.Levonen A-L, Inkala M, Heikura T, Jauhiainen S, Jyrkkänen H-K, Kansanen E,et al. Nrf2 gene transferinduces antioxidant enzymes and suppresses smooth muscle cell growth in vitro and reduces oxidativestress in rabbit aorta in vivo. Arterioscler Thromb Vasc Biol. 2007;27(4):741-7. PMID: 17255530.DOI:http://dx.doi.org/10.1161/01.ATV.0000258868.80079.4d30.Ho HK, White CC, Fernandez C, Fausto N, Kavanagh TJ, Nelson SD, et al. Nrf2 activation involves anoxidative-stress independent pathway in tetrafluoroethylcysteine-induced cytotoxicity. Toxicol Sci.2005;86(2):354-64. PMID: 15901913. DOI:http://dx.doi.org/10.1093/toxsci/kfi20531.Calkins MJ, Johnson DA, Townsend JA, Vargas MR, Dowell JA, Williamson TP, et al. The Nrf2/AREpathway as a potential therapeutic target in neurodegenerative disease. Antioxid Redox Signal.2009;11(3):497-508.PMID: 18717629. PMCID:PMC2933570. DOI:http://dx.doi.org/10.1089/ars.2008.224232.Nakaso K, Yano H, Fukuhara Y, Takeshima T, Wada-Isoe K, Nakashima K. PI3K is a key molecule in theNrf2-mediated regulation of antioxidative proteins by hemin in human neuroblastoma cells. FEBSLett. 2003;546(2):181-4. PMID: 12832036. DOI:http://dx.doi.org/10.1016/S0014-5793(03)00517-9
Kim T-H, Hur E-g, Kang S-J, Kim J-A, Thapa D, Lee YM, et al. NRF2 blockade suppresses colon tumorangiogenesis by inhibiting hypoxia-induced activation of HIF-1α. Cancer Res. 2011;71(6):2260-75.PMID: 21278237. DOI:http://dx.doi.org/10.1158/0008-5472.CAN-10-300734.Deveza L, Choi J, Yang F. Therapeutic Angiogenesis for Treating Cardiovascular Diseases. Theranostics.2012;2(8):801. PMID: 22916079.PMCID:PMC3425124. DOI:http://dx.doi.org/10.7150/thno.441935.Muthusamy VR, Kannan S, Sadhaasivam K, Gounder SS, Davidson CJ, Boeheme C, et al. Acute exercisestress activates Nrf2/ARE signaling and promotes antioxidant mechanisms in the myocardium. FreeRadic Biol Med. 2012;52(2):366-76. PMID: 22051043. PMCID:PMC3800165. DOI:http://dx.doi.org/10.1016/j.freeradbiomed.2011.10.44036.Calvert JW, Jha S, Gundewar S, Elrod JW, Ramachandran A, Pattillo CB, et al. Hydrogen sulfide mediatescardioprotection through Nrf2 signaling. Circ Res. 2009;105(4):365-74. PMID: 19608979. PMCID:PMC2735849. DOI:http://dx.doi.org/10.1161/CIRCRESAHA.109.19991937.Stein AB, Bolli R, Dawn B, Sanganalmath SK, Zhu Y, Wang O-L, et al. Carbon monoxide induces a latepreconditioning-mimetic cardioprotective and antiapoptotic milieu in the myocardium. J Mol CellCardiol. 2012;52(1):228-36. PMID: 22119801. PMCID:PMC3679555. DOI:http://dx.doi.org/10.1016/j.yjmcc.2011.11.00538.GurusamyN, Ray D, Lekli I, Das DK. Red wine antioxidant resveratrol‐modified cardiac stem cellsregenerate infarcted myocardium. J Cell Mol Med. 2010;14(9):2235-9. PMID: 20716127. DOI:http://dx.doi.org/10.1111/j.1582-4934.2010.01140.x39.Gorbunov N, Petrovski G, Gurusamy N, Ray D, Kim DH, Das DK. Regeneration of infarcted myocardiumwith resveratrol‐modified cardiac stem cells. J Cell Mol Med. 2012;16(1):174-84. PMID: 21352470.DOI:http://dx.doi.org/10.1111/j.1582-4934.2011.01281.x40.Ashrafian H, Czibik G, Bellahcene M, Aksentijević D, Smith AC, Mitchell SJ, et al. Fumarate iscardioprotective via activation of the Nrf2 antioxidant pathway. Cell Metab. 2012;15(3):361-71.PMID: 22405071. PMCID: PMC3314920. DOI:http://dx.doi.org/10.1016/j.cmet.2012.01.017
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Knowledge Kingdom Publishing
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
