Histological evaluation of orthodontic tooth movement following low level laser irradiation in rabbits

Authors

  • Hooman Shafaee Oral & Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Keywords:

Low level laser, Tooth movement, Rabbit, Orthodontics

Abstract

Background: Reduction of orthodontic treatment time is a major concern for orthodontists. Low level laser therapy (LLL) has been widely used in dentistry and it has been claimed that it could stimulate bone formation.  Objective: The aim of the present study was to evaluate histological effects of LLL on alveolar bone remodeling during experimental tooth movement in rabbits. Methods: In this experimental study which was performed in the animal lab of Mashhad Dental School in 2016 twenty pre-pubertal female albino rabbits with the mean age of 8±1 weeks were randomly assigned into two groups. All the rabbits were examined by a veterinarian, and underweight or diseased rabbits were excluded from the study. Fifty gram orthodontic force was applied via 0.014 stainless steel spring on central teeth in both groups. In the first group, low level laser KLO3 (wave length: 980 nm, Power: 80mw) was irradiated three minutes from the buccal aspect of central teeth for 21 days. In the second group, rabbits were not exposed to laser irradiation and served as our control group. After this period, the distance between mesial corners of incisors was measured blindly by a caliper calibrated as 0.1mm. All rabbits in both groups were sacrificed by vital perfusion for histological evaluation to determine the number of resorption lacuna and amount of fibrous tissue. Independent sample t-test was used to analyze the data. Results: The number of resorption lacuna, the rate of tooth movement and fibrous tissue were significantly greater in the lased group (p=0.05). Conclusion: Low level laser irradiation could accelerate tooth movement in rabbits via increased resorptive activity in alveolar bone.

 

References

Lim HM, Lew KK, Tay DK. A clinical investigation of the efficacy of low level laser therapy in reducingorthodontic post adjustment pain. Am J Orthod DentofacialOrthop. 1995; 108: 614–22. doi:10.1016/S0889-5406(95)70007-2.

Abtahi M, Shafaee H, Saghravania N, Peel S, Giddon D, Sohrabi K. Effect of corticosteroids onorthodontic tooth movement in a rabbit model. J Clin Ped Dent. 2014; 38(3): 285-9. doi:10.17796/jcpd.38.3.n04h7105121663t6. PMID: 25095326.3)Mirhashemi AH, Akhoundi MS, Sheikhzadeh S, Momeni N, Dehpour A, Alaeddini M, et al. Effect offluoxetine consumption on orthodontic tooth movement in rats. J Dent. 2015; 12(12): 882-9.4)Abtahi SM, Mousavi SA, Shafaee H, Tanbakuchi B. Effect of low-level laser therapy on dental paininduced by separator force in orthodontic treatment. Dent Res J (Isfahan). 2013; 10(5): 647-51. PMID:24348624, PMCID: PMC3858741.5)Tang XM, Chai BP. Effects of CO2 laser irradiation on experimental fracture healing: a transmissionelectron microscopic study. Lasers Surg Med. 1986; 6: 346–52. doi: 10.1002/lsm.1900060309. PMID:3090390.6)Trelles MA, Mayayo E .Bone fracture consolidates faster with low-power laser. Lasers Surg Med. 1987;7:36–45. doi: 10.1002/lsm.1900070107. PMID: 3573935.7)Barushka O, Yaakobi T, Oron U. Effect of low-energy laser (He.Ne) irradiation on the process of bonerepair in the rat tibia. Bone. 1995; 16: 47–55. PMID: 7742082.8)Saito S, Shimizu N. Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatalsuture during expansion in the rat. Am J Orthod Dentofacial Orthop. 1997; 111: 525–32. doi:10.1016/S0889-5406(97)70152-5. PMID: 9155812.9)Guzzardella GA, Fini M, Torricelli P, Giavaresi G,Giardino R. Laser stimulation on bone defect healing:An In Vitro Study. Lasers Med Sci. 2002; 17: 216–20. doi: 10.1007/s101030200031. PMID: 12181636.10)Ozawa Y, Shimizu N, Kariya G, Abiko Y. Low-energy laser irradiation stimulates bone nodule formationatearly stages of cell culture in rat calvarial cells. Bone. 1998; 22: 347–54. doi: 10.1016/S8756-3282(97)00294-9. PMID: 9556134.11)Yamaguchi M, Fujita S, Yoshida T, Oikawa K, Yamamoto H, Kasai K. Low-energy laser irradiationstimulates the tooth movement velocity via expression of M-CSF and c-fms. Orthod Waves. 2007; 66: 139–48. doi: 10.1016/j.odw.2007.09.002.12)Fujita S, Yamaguchi Y, Utsunomiya T, Yamamoto H, Kasai K. Low-energy laser irradiation stimulatestooth movement velocity via expression of RANK and RANKL. Orthod Craniofac Res. 2008; 11: 143–55.doi: 10.1111/j.1601-6343.2008.00423.x. PMID: 1871315113)Kawasaki K, Shimizu N. Effects of low-energy laser irradiation on bone remodeling during experimentaltooth movement in rats. Lasers Surg Med. 2000; 26: 282–91. doi: 10.1002/(SICI)1096-9101(2000)26:3<282::AID-LSM6>3.0.CO;2-X. PMID: 10738291.14)Akin E, Gurton AU, Olmez H. Effects of nitric oxide on orthodontic tooth movement in rats. Am J OrthodDentofacial Orthop. 2004; 126(5): 608-14. doi: 10.1016/S0889540604004494. PMID: 15520694.15)Poosti M, Basafa M, Eslami N. Progesterone effects on experimental tooth movement in rabbits. J CalifDent Assoc. 2009; 37: 483-6. PMID: 19653405.16)Youssef M, Ashkar S, Hamade E, Gutknech N, Lomper TF, Mir M. The effect of low level laser therapyduring orthodontic movement: a preliminary study. Lasers Med Sci. 2007; 23: 27-33. PMID: 17361391.17)Cruz DR, Kohata EK, Ribeiro MS, Wetter NU. Effects of low intensity laser therapy on the orthodonticmovement velocity of human teeth: a preliminary study. Lasers Surg Med. 2004; 35: 117-20. doi:10.1002/lsm.20076. PMID: 15334614.18)Limpanichkul W, Godfrey K, Srisuk N, Rattanayatikul C. Effects of low-level laser therapy on the rate oforthodontic tooth movement. Orthod Craniofac Res. 2006; 9: 38-43.doi: 10.1111/j.1601-6343.2006.00338.x. PMID: 16420273.19)Kim YD, Song WW, Kim SS, Kim GC, Hwang DS, Shin SH, et al. Expression of receptor of nuclearfactor-KB ligand, receptor activator of nuclear factor-KB, and osteoprotegerin, following low-level lasertreatment on deproteinized bovine bone graft in rats. Lasers Med Sci. 2009; 24: 577-84. doi:10.1007/s10103-008-0614-7. PMID: 18825474.20)Seifi M, Shafeei HA, Daneshdoost S, Mir M. Effects of two types of low level laser wave lengths (850 and630 nm) on the orthodontic tooth movements in rabbits. Lasers MedSci. 2007; 22: 261-4. doi:10.1007/s10103-007-0447-9. PMID: 17334676.21)Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, Chapple CC, et al. The effect of low levellaser on osteoblastic cells. Clin Orthod Res. 2001; 1: 3–14. PMID: 11553080.22)Song CW, Chelstrom LM, Levitt SH, Haumschild DJ. Effects of temperature on blood circulationmeasured with the laser Doppler method. Int J Radiat Oncol Biol Phys. 1989; 17: 1041-7. doi:10.1016/0360-3016(89)90153-3.PMID: 2808037

Published

2021-12-24