The antimicrobial activity of probiotic bacteria Escherichia coli isolated from different natural sources against hemorrhagic E. coli O157:H7
Keywords:
Probiotics, Enteropathogenesis, Enterohemorrhagic Escherichia coli, Escherichia coli O157:H7Abstract
Background: Diarrheal diseases have been seen in all geographical areas throughout the world. Therefore, considering treatment, could be deemed a necessary action. Objective: The aim of this study was to determine the antimicrobial effect of probiotic bacterial strains isolated from different natural sources against 2 pathotypes of pathogenic E. coli. Methods: This cross-sectional study of Martyr Chamran University of Ahvaz was carried out from December 2013 to July 2014. A total of 13 probiotic colonies isolated from 20 samples of traditional dairy products including (yogurt, cheese, milk) and 20 samples of vegetables including carrots and cabbages (red and white) of which 5 isolates were selected to evaluate the antimicrobial effect against 2 Escherichia coli pathotypes, randomly. Antimicrobial effect was evaluated using two methods: disk diffusion and well diffusion tests and measuring growth inhibition zones of probiotics against 2 pathotypes of pathogenic E. coli. Results: Obtained results showed growth inhibition effects of all 5 probiotic strains against Escherichia coli pathotypes in both used methods. All selected strains showed considerable antimicrobial effect on Escherichia coli O157:H7 strain, but had no inhibitory effect against Enterohemorrhagic Escherichia coli. Conclusion: This study demonstrated considerable antimicrobial effect against E. coli O157:H7 strain. Due to this, characteristic and similar antimicrobial effects of probiotics bacteria, increasing use of the probiotics as a natural and modern method for prevention of different diseases is recommended.
References
Soccol CR, Vandenberghe LP, Spier MR, Medeiros AB, Yamaguishi CT, Lindner JD, et al. The potential
of probiotics: a review. Food Technology and Biotechnology. 2010; 48(4): 413-34.
Anandharaj M, Sivasankari B, Parveen Rani R. Effects of probiotics, prebiotics, and synbiotics on
hypercholesterolemia: a review. Chinese Journal of Biology. 2014; 2014(1): 7. doi: 10.1155/2014/572754.
Torkan S, Shirani D, Rahimi E, Ghomi K. The Evaluation of Probiotic Effect on Prevention of Food
Bacterial Poisoning in Dog. J of Comparative Pathobiology. 2011; 8(1): 403-13.
Taniuchi M, Walters CC, Gratz J, Maro A, Kumburu H, Serichantalergs O, et al. Development of a
multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its
evaluation on colonies, culture broths, and stool. Diagn Microbiol Infect Dis. 2012; 73(2): 121-8. doi:
1016/j.diagmicrobio.2012.03.008. PMID: 22541788.
Nahaei MR, Akbari Dibavar M, Sadeghi J, Nikvash S. Frequency of enterohaemorrhagic Escherichia
coliisolated from patients with acute diarrhea in Tabriz hospitals. Iran J Med Microbiol. 2007; 1(3): 39-46.
Center for Disease Control. Escherichia coli O157: H7, 2001.
Combs BG, Raupach JC, Kirk MD. Surveillance of Shiga toxigenic Escherichia coli in Australia. Commun
Dis Intell Q Rep. 2005; 29(4): 366-9. PMID: 16465925.
Sieladie DV, Zambou F, Kaktcham PM, Cresci A, Fonteh F. Probiotic properties of lactobacilli strains
isolated from raw cow milk in the western highlands of Cameroon. Innovative Romanian Food
Biotechnology. 2011; 9: 12.
Nataro JP, Mai V, Johnson J, Blackwelder WC, Heimer R, Tirrell S, et al. Diarrheagenic escherichia coli
infection in Baltimore, Maryland, and New haven, Connecticut. Clin Infect Dis. 2006; 43(4): 402-7. doi:
1086/505867. PMID: 16838226.
Erdoğan H, Levent B, Erdoğan A, Güleşen R, Arslan H. [Investigation of verotoxigenic Escherichia coli
O157: H7 incidence in gastroenteritis patients]. Mikrobiyol Bul. 2011; 45(3): 519-25. PMID: 21935785.
Salaj R, Štofilová J, Šoltesová A, Hertelyová Z, Hijová E, Bertková I, et al. The effects of two
Lactobacillus plantarum strains on rat lipid metabolism receiving a high fat diet. The Scientific World
Journal. 2013; 2013(1): 7. doi: 10.1155/2013/135142.
Darsanak KR, Ghaemi N, Mirdavoodi F. Anti-mutation activity of probiotic strains isolated from probiotic
products. J of Qom Uni of Med Sci. 2012; 6(2): 37-44.
Alikhani MY, Mirsalehian A, Fatollahzadeh B, Pourshafie MR, Aslani MM. Prevalence of
enteropathogenic and Shiga toxin-producing Escherichia coli among children with and without diarrhoea in
Iran. J Health Popul Nutr. 2007; 25(1): 88-93. doi: 10.1016/s0924-8579(07)72044-7. PMID: 17615908,
PMCID: PMC3013268.
Enayat K, Fariborz S, Heiman S, Mehdi SD. Frequency, antimicrobial susceptibility and plasmid profiles of
Escherichia coli pathotypes obtained from children with acute diarrhea. Jundishapur Journal of
Microbiology. 2011; 4(1): 23-8.
Jertborn M, Svennerholm AM. Enterotoxin-producing bacteria isolated from Swedish travellers with
diarrhoea. Scand J Infect Dis. 1991; 23(4): 473-9. doi: 10.3109/00365549109075096. PMID: 1957131.
Baudry B, Savarino SJ, Vial P, Kaper JB, Levine MM. A sensitive and specific DNA probe to identify
enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis. 1990; 161(6):
-51. doi: 10.1093/infdis/161.6.1249. PMID: 2189007.
Sarshar M, Tavafi H, Ghorbani DS, Sood N, Kargar M, Shahrokhi N. Antibiotic resistance to tetracycline
resistance genes in strains of E. coli and distribution diarrhea isolated from children. Iranian Journal of
Infectious Diseases. 2012; 17(57): 13-8.
Zeighami H, Haghi F, Hajiahmadi F, Kashefiyeh M, Memariani M. Multi-drug-resistant enterotoxigenic
and enterohemorrhagic Escherichia coli isolated from children with diarrhea. J Chemother. 2014; 27(3):
-5. doi: 10.1179/1973947813Y.0000000161. PMID: 24571245.
Pourakbari B, Heydari H, Mahmoudi S, Sabouni F, Teymuri M, Ferdosian F, et al. Diarrhoeagenic E. coli
pathotypes in children with and without diarrhoea in an Iranian referral paediatrics centre. East Mediterr
Health J. 2013; 19(7): 617-21. PMID: 24975306.
Kargar M, Homayon M, Yaghoubi R, Manokianis A. Pathogenic gene stx1, stx2, eaeA and hly with
Multiplex PCR in strains of E. coli O157: H7 isolated from children with acute gastroenteritis in the city of
Shiraz. Iranian Journal of Infectious Diseases. 2009; 14(44): 7-12.
Hassanzadazar H, Ehsani A, Mardani K. Antibacterial activity of Enterococcus faecium derived from
Koopeh cheese against Listeria monocytogenes in probiotic ultra-filtrated cheese. Vet Res Forum. 2014;
(3): 169-75. PMID: 25568714, PMCID: PMC4279643.
Hassanzadazar H, Ehsani A, Mardani K, Hesari J. Investigation of antibacterial, acid and bile tolerance
properties of lactobacilli isolated from Koozeh cheese. Vet Res Forum. 2012; 3(3): 181-5. PMID:
, PMCID: PMC4299980.
Mehrabian S, Tahmasbi H, Momtaz H, Farahmandi SH, Davoodi JH. Find carrying E. coli Shiga toxin
genes of Multiplex Polymerase Chain Reaction method for Escherichia coli isolated from patients with
acute diarrhea referred to Hajar hospital. 2012; 18(21): 8-14.
Lotfalie Haghighi. Intestinal bacteria (Enterobacteriaceae family). Press start treatment Bushehr University
of Medical Sciences. 2004; 10-20.
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