Bioactive molecule of essential oil of Cuperssus sempervirence Mill – in vitro other treatment against microbial pathogenesis

Abstract

Background: Each year, many million people attract infections caused by bacteria, fungi, virus,
and parasite, infectious diseases reached at high rate, and the problem of emerging and reemerging
infections becomes a serious danger to human health, medicinal plants constitute a
source of bioactive molecules can fight against infection microbial disease. In the context of this
research, was intended to characterize biomolecule of hydro-distilled essential oil of Cuperssus
sempervirence produces in west of Algeria and its potentials against disease infections.
Methods: The essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS)
method and Antibacterial activity was investigated in vitro against twelve clinical and reference
encountered in Clinical Laboratory and the Microbiology Laboratory of Saidal Medea using the
well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal
concentration (MBC) were determined by the dilution method agar.
Results: The most common components usually found in cypress essential oils were present in the
oil samples analyzed, 43 components, which constitute 97,893% were identified in the essential
oil. The major constituents were Alpha pinene (60.05%), delta-3-carene (18.08%) et terpinolene
(2.44%). myrcene (2.39%), limonene (1.83%), the results of the disk diffusion method showed
high essential oils activity against all strains tested . The best antimicrobial activity against
Candida albicans ATCC1231was found (0.001v/v) .Bactericidal activity was higher for EO
(minimal inhibitory concentration (MICs) 0.02(v/v)) against almost all tested bacteria, except
multi drug resistant strains of Salmonella typhimiruim ATCC13311 and Bacillus cereus
ATCC10876. The results obtained of this study provide that essential oil of Cuperssus
sempervirence is treatment against microbial pathogenesis human.