Filed studies on some probiotics to minimize hazard effects of prevailing heavy metals contamination for improving immunity and growth performance of Oreochromis niloticus

Authors

  • Mona Saad Zaki Department of Hydrobiology, Veterinary Division, National Research Center, Cairo, Egypt

Keywords:

Probiotic, L. acidophilus, Cadmium, Growth performance, Macronucleus

Abstract

Aim: The objective of the present study was to determine the effect of some types of probiotics to minimize the hazard effect of cadmium on Oreochromis niloticus (O. niloticus) and to determine the influence of probiotics on the growth performance and immune status of O. niloticus. 

Methods: Fish were divided into four groups, the first one was fed on a diet without any additives (control group), and the second group was fed on diet with the addition of 0.1 from (LC50) of cadmium 32 ppm in water. The third group was fed on a diet containing 1 gm/kg of Lactobacillus acidophilus. The fourth group was fed on a diet containing 1 gm/kg of L. acidophilus with 0.1 addition from (LC50) of cadmium 32 ppm.

Results: The experiment lasted about 60 days which resulted in an improvement in growth performance, with a decrease in the number of micronucleus formation in the group which received the probiotic. The group which was affected with cadmium showed decrease in the growth performance with increase in the number of micronucleus formation while the group which received probiotics and treated with cadmium had significant improvement in growth performance and decreased in the number of micronucleus formation.

Conclusions: Probiotics decrease cadmium pollution.

References

Kalay M, Canli M. Elimination of essential (Cu and Zn) and non-essential (Cd and Pb) metals from tissues

of a fresh water fish, Tilapia zillii. Tropical Journal of Zoology. 2000; 24: 429-36.

Olaifa FE, Olaifa AK, Adelaja AA, Owolabi AG. Heavy metal contamination of Clariasgarpinus from a

lake and Fish farm in Ibadan, Nigeria. Afric J of Biomed Res. 2004; 7: 145-8. doi:

4314/ajbr.v7i3.54185.

Khaled A. Heavy metal concentrations in certain tissues of five commercially important fishes from El- Mex Bay, Al-Exandria, Egypt. Egyptian Journal of Aquatic Biology and Fisheries. 2004; 8(1): 51-64.

Ashmawy AA, Rashed MA, Atta AH, Ibrahim AG, Abdel-Gawad FK. Genotoxic Effect of Cadmium on

NileTilapia (Oreochromis niloticus). International Journal of Scientific & Engineering Research. 2015;

(8).

Choudhury HT, Harvey WC, Thayer TF, Lockwood WM, Stiteler PE, Goodrum JM, et al. Real-time PCR.

EnfermInfecc Microbiol Clin. 2004; 22(5): 299-304.

Teemu H, Seppo S, Jussi M, Raija T, Kalle L. Reversible surface binding of cadmium and lead by lactic

acid and bifidobacteria. Int J Food Microbiol. 2008; 125(2): 170-5. doi: 10.1016/j.ijfoodmicro.2008.03.041.

PMID: 18471917.

El Zokm GM, Okbah MA, Younis AM. Assessment of heavy metals pollution using AVS-SEM and

fractionation techniques in Edku Lagoon sediments, Mediterranean Sea, Egypt. J Environ Sci Health A Tox

Hazard Subst Environ Eng. 2015; 50(6): 571-84. doi: 10.1080/10934529.2015.994945. PMID: 25837560.

Joshi J, Sahu O. American Public Health Association. Standard Methods for the Examination for Water and

Wastewater. 19th edition. Byrd Prepess Springfield; 1995.

Grisolia CK, Cordeiro CMT. Variability in micronucleus induction with different mutagens applied to

several species of fish. Gen Mol Biol. 2000; 23(1): 235. doi: 10.1590/S1415-47572000000100041.

Dimari GA, Abdulrahman JC, Garba ST. Metals concentrations in tissues of Tilapia galli, Clariaslazera and

Osteoglossidae caught from Alau Dam, Maiduguri, Borno State, Nigeria. American Journal of

Environmental Sciences. 2008; 4(4): 373-9. doi: 10.3844/ajessp.2008.373.379.

W H O. Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic

Acid Bacteria. World Health Organization, Geneva. 2001.

Tapia-Paniagua S, Lobo C, Moreno-Ventas X, Banda IG, Moriñigo MA, Carmen Balebona M. Probiotic

Supplementation Influences the Diversity of the Intestinal Microbiota During Early Stages of Farmed

Senegalese Sole (Solea senegalensis, Kaup 1858). Marine Biotechnology. 2014; 16(6): 716-28. doi:

1007/s10126-014-9588-6.

Kaoud HA, Zaki MM, El-Dahshan AR, Saeid Sh, El Zorba HY. Amelioration the Toxic Effects of

Cadmium-Exposure in Nile Tilapia (Oreochromis Niloticus) by using Lemnagibba L. Life Science Journal.

; 8(1): 185-95.

Garcia Santos S, Fontaínhas-Fernandes A, Wilson JM. Cadmium tolerance in the Nile tilapia (Oreochromis

niloticus) following acute exposure: assessment of some ionoregulatory parameters. Environ Toxicol. 2006;

(1): 33-46. doi: 10.1002/tox.20152. PMID: 16463259.

Marzouk MS, Mostafa MM, Mohamed NM. Evaluation of immunomodulatory effect of some probiotics on

cultured oreochromis niloticus. 8th International Symposium on Tilapia in Aquaculture. 2008; 1043-58.

Siliem NA. Rearing studies on Oreochromis niloticus to evavaluate bacillus subtilis potential in growth

performance and some physiological parameters. Egypt J Aquat Biol & Fish. 2008; 12(4): 1-11.

Hrnandez LLH, Barrera TC, Mejia GC, Mejia JC, Carmen MD, Dosta M, et al. Effects of the commercial

probiotic Lactobacillus casei on the growth, protein content of skin mucus and stress resistance of juveniles

of the Porthole livebearer Poecilopsisgracilis (Poecilidae). Aquaculture Nutrition. 2010; (16): 407–11. doi:

1111/j.1365-2095.2009.00679.x.

Al-Dohai MA, Hashim R, Aliyu Paiko M. Effects of the probiotic, Lactobacillus acidophilus, on the growth

performance, haematology parameters and immunoglobulin concentration in African Catfish

(Clariasgariepinus, Burchell 1822) fingerling. Aquaculture Research. 2009; 40(14): 1642–52. doi:

1111/j.1365-2109.2009.02265.x.

Ridha MT, Azad IS. Preliminary evaluation of growth performance and immune response of Nile tilapia

Oreochromis niloticus supplemented with two putative probiotic bacteria. Aquaculture Research. 2012;

(6): 843–52. doi:10.1111/j.1365-2109.2011.02899.x.

Lara-Flores M, Olvera-Novoa MA, Guzmán-Méndez BE, Lopez-Madrid W. Use of the bacteria

Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth

promoters in Nile tilapia (Oreochromis niloticus). Aquaculture. 2003; 216(1-4): 193-201. doi:

1016/S0044-8486(02)00277-6.

Monachese M, Burton JP, Reid G. Bioremediation and tolerance of humans to heavy metals through

microbial processes: a potential role for probiotics? Appl Environ Microbiol. 2012; 78(18): 6397-404. doi:

1128/AEM.01665-12. PMID: 22798364, PMCID: PMC3426676.

Ayoola SO, Ajani EK, Fashae OF. Effect of Probiotics (Lactobacillus and Bifidobacterium) on Growth

Performance and Hematological Profile of Clarias gariepinus Juveniles. World Journal of Fish and Marine

Sciences. 2013; 5(1): 1-8. doi: 10.5829/idosi.wjfms.2013.05.01.6582.

Russo C, Rocco L, Morescalchi MA, Stingo V. Assessment of environmental stress by the micronucleus

test and the Comet assay on the genome of teleost populations from two natural environments. Ecotoxicol

Environ Saf. 2004; 57: 168–74. doi: 10.1016/S0147-6513(03)00027-7. PMID: 14759663.

Mahrous KF, Hassan AM, Radwan HA, Mahmoud MA. Inhibition of cadmium- induced genotoxicity and

histopathological changes in Nile tilapia fish by Egyptian and Tunisian montmorillonite clay. Ecotoxicol

Environ Saf. 2015; 119: 140-7. doi: 10.1016/j.ecoenv.2015.04.054. PMID: 26001163.

Jindal R, Verma S. In vivo genotoxicity and cytotoxicity assessment of cadmium chloride in peripheral

erythrocytes of Labeo rohita (Hamilton). Ecotoxicol Environ Saf. 2015; 118: 1-10. doi:

1016/j.ecoenv.2015.04.005. PMID: 25890049.

Published

2022-01-18

Issue

Section

Articles