A Survey of the quantity and type of biological aerosols in selected wards of a teaching hospital in Ghazvin

Authors

  • Fatemeh Karimi Department of Environmental Health Engineering, School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Keywords:

bioaerosol, hospital, air-conditioning, indoor air

Abstract

Introduction: Bioaerosols are agents that can cause infection, allergy or induce other toxic effects in the human body. If the person exposed to such particles is not capable of their destruction or elimination from the body, the established chemical and physiological disorders can result in disease or death. The aim of this study was to assess the concentrations of bioaerosols in several wards of a teaching hospital.

Methods: Given that gas air-conditioners (split and window types) were used for ventilation in the eye operating room, internal intensive care unit, and the respiratory isolation room, these wards were selected for passive sampling. Sterile plates containing culture medium were exposed for two hours to the wards’ indoor ambient air. After this time, they were transferred to a lab to undergo incubation, colony count, and identification of the microorganisms. The data were analyzed using SPSS software, version 18, and the significance level of less than 0.05 was used. 

Results: Based on our findings, the highest colony-forming bacterial unit was observed 22 cfu/plate/h in the eye operating room and, the highest colony-forming fungal unit was observed 4 cfu/plate/h in the internal intensive care unit. Based on the results of the differential tests, the most prevalent bacteria identified were Staphylococcus epidermidis (75%) in the air of eye operating room and Staphylococcus saprophyticus (52%) in the internal intensive care unit and isolation room. The most prevalent identified fungi in the air of selected wards were related to Alternaria alternata (43%), Aspergillus flavus (24%), Penicillium (36%) and Curvularia (21%) types. Based on Spearman's correlation test, no significant relationship was observed between the factor of temperature and the number of fungal and bacterial colonies (r = 0.201, p = 0.42; r = -0.197, p = 0.41). Moreover, a meaningful relationship was observed only between the number of individuals and the bacterial colonies present in the air (r = 0.749, p = 0.0001).

Conclusion: Comparing the number of diagnosed colonies by 25 cfu/plate/h value showed that the numbers of colonies in all samples were lower than the mentioned values. So, the air-conditioning systems’ performance in reducing the number of bioaerosols in the studied wards was satisfactory. However, since the remaining bacterial and fungal contamination can cause serious infections in patients, it is highly necessary that the cleaning intervals and replacement time points of such filters be programmed based on the number of individuals present in the wards and their active surgical hours.

References

Chubineh AR, Rostami R, Tabataabae SHR. Assessment of Bioaerosols Types and Concentration in

Ambient Air of Shiraz University of Medical Sciences Educational Hospitals in 2008. Iran Occupational

Health. 2009; 6(2): 69-76.

Hoseinzadeh E, Taghavi M, Samarghandie MR. Evaluation of Fungal and Bacterial Aerosols in The

Different Wards of Malayer City’s Hospitals in 2011-2012. Hospital. 2014; 13(3): 99-108.

Griffiths W., Decosemo GAL. The Assessment of Bioaerosols: A Critical Review. Journal of Aerosol

Science. 1994; 25(8): 1425-58. doi: 10.1016/0021-8502(94)90218-6.

Setlhare G, Malebo N, Shale K, Lues R. Identification of Airborne Microbiota in Selected Areas in a

Health-Care Setting in South Africa. BMC Microbiol. 2014; 14: 100. doi: 10.1186/1471-2180-14-100,

PMID: 24750818, PMCID: PMC4016773.

Napoli C, Marcotrigiano V, Montagna MT. Air Sampling Procedures to Evaluate Microbial Contamination:

A Comparison Between Active and Passive Methods in Operating Theatres. BMC Public Health. 2012; 12:

doi: 10.1186/1471-2458-12-594, PMID: 22853006.

Memarzadeh F, Xu W. Role of Air Changes per Hour (ACH) in Possible Transmission of Airborne

Infections. Journal of Build Simul. 2012; 5: 15-28. doi: 10.1007/s12273-011-0053-4.

Jung CC, Wu PC, Tseng CH, Su HJ. Indoor Air Quality Varies with Ventilation Types and Working

Areasin Hospitals. Journal of Building and Environment. 2015; 85: 190-5. doi:

1016/j.buildenv.2014.11.026.

Beggs CB, Kerr KG, Noakes CJ, Hathway EA, Sleigh PA. The ventilation of multiple-bed hospital wards:

Review and analysis. Am Journal of Infection Control. 2008; 36(4): 250-9. doi: 10.1016/j.ajic.2007.07.012,

PMID: 18455045.

Nourmoradi H, Nikaeen M, Amin MM, Hatamzadeh M. An Investigation on Bio-aerosol Concentrations in

the Different Wards of Hospitals of Isfahan University of Medical Sciences. Journal of Isfahan Medical

School. 2011; 29(149): 1028.

Abdollahi AR. Concurrence of Nosocomial Infections with Microorganisms Spreading in the Air of

Hospital Wards. Medical laboratory Journal. 2010; 3(2): 47-52.

Azizifar M, Jabari H, Nadafi K, Nabizadeh R, Tabaraei Y, AzamSolgi A. A Qualitative and Quantitative

Survey on Air-Transmitted Fungal Contamination in Different Wards of Kamkar Hospital in Qom Iran in

Qom University of Medical Sciences Journal. 2009; 3(3): 25-30.

Chow TT, Yang XY. Ventilation Performance in Operating Theatres Against Airborne Infection: Review

of Research Activities and Practical Guidance. Journal of Hospital Infection. 2004; 56: 85-92. doi:

1016/j.jhin.2003.09.020, PMID: 15019218.

Wan GH, Chung PP, Tang CS. Long-term Surveillance of Air Quality in Medical Center Operating Rooms.

Am J Infect Control. 2011; 39(4): 302-8. doi: 10.1016/j.ajic.2010.07.006, PMID: 21256628.

Khawcharoenporn T, Apisarnthanarak A, Thongphubeth K, Yuekyen C, Damnin S, Hayden MK, et al.

Post-Flood Measurment of Fungal Bio-Aerosol in a Resource-Limited Hospital: Can The Settle Plate

Method Be Used?. Journal of Hospital Infection. 2013; 83: 150-2. doi: 10.1016/j.jhin.2012.11.012.

Masoumbeigi H, Esmaeili D, yamchi H, Sepandi M. Survey of Air Bacteriological Contamination Rate in

One of the Hospital's Dentistry Centers in Tehran. Iran Journal of Med Microbio. 2015; 9(2): 73-8.

Mirbahar AM, Memon BA. Bacteriological Monitoring through Air Sampling in Different Locations of

Teaching/Civil Hospital Sukkur. Journal of App Em Sc. 2005; 1(2): 13-5.

Verde SC, Almeida SM, Matos J, Guerreiro D, Meneses M, Faria T, et al. Microbiological Assessment of

Indoor Air Quality at Different Hospital Sites. Res Microbiol. 2015; 166(7): 557-63. doi:

1016/j.resmic.2015.03.004, PMID: 25869221.

Noorian AA, Badali H. Studying the fungi contaminating the air, the operating room equipment and certain

wards of Zanjan city hospitals. Journal of Zanjan University of Medical Sciences and Health Services.

; 36: 9-16.

Published

2022-03-07

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