Reduction of Dacarbazine cytogenetic effects on somatic cells in male mice using bee glue (Propolis) to manifest the scientific miracles in the Quran
Keywords:
Propolis, Dacarbazine, Bone marrow cells, Cytogenetic, MiceAbstract
Objective: This study was carried out to investigate the ability of Propolis to ameliorate the adverse cytogenetic effects of Dacarbazine on bone marrow cells
Methods: In this experimental in vivo study, 18 mice were used, divided into four groups: control group; Propolis-treated group (treated with 50mg/kg Propolis); and Dacarbazine-treated group (treated with 3.5mg/kg Dacarbazine). The fourth, fifth, and sixth were treated with Dacarbazine and Propolis as pre 2h, post 2h, and concomitant treatment. After five days, the Bone Marrow (BM) samples were obtained for cytogenetic investigation.
Results: The in vivo studies revealed that Dacarbazine induced an abnormalities in polychromatic erythrocytes cells (PECs) as increase of cell with micronuclei, while the dual treatment accompanied with improvement of this abnormalities.
Conclusions: It could be concluded that there are protective effects of Propolis against the adverse effects of Dacarbazine. It could be recommended to use Propolis as an adjuvant with chemotherapeutic agents.
References
Ren J, Singh BN, Huang Q, Li Z, Gao Y, Mishra P, et al. DNA hypermethylation as a chemotherapy target.
Cell Signal. 2011; 23(7): 1082-93. doi: 10.1016/j.cellsig.2011.02.003. PMID: 21345368.
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal
physiological functions and human disease. Int J Biochem Cell Biol. 2007; 39(1): 44-84. doi:
1016/j.biocel.2006.07.001. PMID: 16978905.
Silverman RB, Holladay MW. The Organic Chemistry of Drug Design and Drug Action, 3rd Edition.
Academic press. 2014.
Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition. 2002; 18(10): 872-9. doi:
1016/S0899-9007(02)00916-4. PMID: 12361782.
Gerald D, Berra E, Frapart YM, Chan DA, Giaccia AJ, Mansuy D, et al. JunD reduces tumor angiogenesis
by protecting cells from oxidative stress. Cell. 2004; 118(6): 781-94. doi: 10.1016/j.cell.2004.08.025. PMID: 15369676.
Mena S, Ortega A, Estrela JM. Oxidative stress in environmental-induced carcinogenesis. Mutat Res. 2009;
(1-2): 36-44. doi: 10.1016/j.mrgentox.2008.09.017. PMID: 18977455.
Elsendoorn TJ, Weijl NI, Mithoe S, Zwinderman AH, Van Dam F, De Zwart FA, et al. Chemotherapyinduced chromosomal damage in peripheral blood lymphocytes of cancer patients supplemented with
antioxidants or placebo. Mutat Res. 2001; 498(1-2): 145-58. doi: 10.1016/S1383-5718(01)00278-9. PMID:
da Rocha AB, Lopes RM, Schwartsmann G. Natural products in anticancer therapy. Curr Opin Pharmacol.
; 1(4): 364-9. doi: 10.1016/S1471-4892(01)00063-7. PMID: 11710734.
Conklin KA. Cancer chemotherapy and antioxidants. J Nutr. 2004; 134(11): 3201-4. PMID: 15514307.
Weijl NI, Elsendoorn TJ, Lentjes EG, Hopman GD, Wipkink-Bakker A, Zwinderman AH, et al.
Supplementation with antioxidant micronutrients and chemotherapy-induced toxicity in cancer patients
treated with cisplatin-based chemotherapy: a randomised, double-blind, placebo-controlled study. Eur J
Cancer. 2004; 40(11): 1713-23. doi: 10.1016/j.ejca.2004.02.029. PMID: 15251161.
Shen DW, Pouliot LM, Hall MD, Gottesman MM. Cisplatin resistance: a cellular self-defense mechanism
resulting from multiple epigenetic and genetic changes. Pharmacol Rev. 2012; 64(3): 706-21. doi:
1124/pr.111.005637. PMID: 22659329, PMCID: PMC3400836.
Drisko JA, Chapman J, Hunter VJ. The use of antioxidant therapies during chemotherapy. Gynecol Oncol.
; 88(3): 434-9. doi: 10.1016/S0090-8258(02)00067-7. PMID: 12648599.
Reda H., Azza A., and Nahla S. Protective role of Propolis ageinst reproductive toxicity of chlorpyrifos in
male rats. Pesticide Biochemistry and Physiology,2011; 101(3): 175-81, doi: 10.1016/j.pestbp.2011.09.003
Hardman, J.G, Limbird, L.E. and Gilman, A.G. Goodman and Gilman's the pharmacological basis of
therapeutics, 2006, 10Ed.
Anton E. Ultrastructural Changes of Stromal Cells of Bone Marrow and Liver After Cyclophosphamide
Treatment in Mice. Tissue Cell. 1997; 29(1): 1-9. doi: 10.1016/S0040-8166(97)80066-3. PMID: 9061976.
Sakr SA, Zoil Mel-S, El-Shafey SS. Ameliorative Effect of Grapefruit Juice on Amiodarone-Induced
Cytogenetic and Testicular Damage in Albino Rats. Asian Pac J Trop Biomed. 2013; 3(7): 573-9 doi:
1016/S2221-1691(13)60116-1. PMID: 23836512, PMCID: PMC3695585.
Heddle JA. A rapid in vivo test for chromosomal damage. Mutat Res. 1973; 18(2): 187-90. PMID:
Serpeloni JM, Bisarro dos Reis M, Rodrigues J, Campaner dos Santos L, Vilegas W, Varanda EA, et al. In
vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet
assay and micronucleus test. Mutagenesis. 2008; 23(6): 501-7. doi: 10.1093/mutage/gen043. PMID:
Gebel T, Lantzsch H, Plessow K, Dunkelberg H. Genotoxicity of platinum and palladium compounds in
human and bacteria cells. Mutat Res. 1997; 389(2-3): 183-90. doi: 10.1016/S1383-5718(96)00145-3ax.
PMID: 9093382.
Kurdi L. The ability of Honey Bee to alleviate the histological, cytological and cytogenetic effects of
cisplatin in mice. M. SC. thesis Submitted to Grils College of Education, Zoolgy Department, Jeddah,
Kingdom of Saudi Arabia. 2000s.
Karpova GV, Fomina TI, Voronova OL, Abramova EV, Loskutova OP. Early and delayed effects of
carboplatin on the blood system. Bull Exp Biol Med. 2001; 132(5): 1065-9. doi:
1023/A:1017964407835. PMID: 11865323.
Al-Etaby MK, Abou-Tarboush FM. Clastogenic effects of carboplatin on SWR/J mouse bone marrow cells.
Arab J Biotech. 2004; 7(1): 75-82.
Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, et al. Molecular mechanisms of
micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells.
Mutagenesis. 2011; 26(1): 125-32. doi: 10.1093/mutage/geq052. PMID: 21164193.
Grover IS, Kaur S. Genotoxicity of wastewater samples from sewage and industrial effluent detected by the
Allium root anaphase aberration and micronucleus assays. Mutat Res. 1999; 426(2): 183-8. doi:
1016/S0027-5107(99)00065-2. PMID: 10350595.
Krishna G, Hayashi M. In vivo rodent micronucleus assay: protocol, conduct and data interpretation. Mutat
Res. 2000; 455(1-2): 155-66. doi: 10.1016/S0027-5107(00)00117-2. PMID: 11113474.
Bakare AA, Okunola AA, Adetunji OA, Jenmi HB. Genotoxicity assessment of a pharmaceutical effluent
using four bioassays. Genet Mol Biol. 2009; 32(2): 373-81. doi: 10.1590/S1415-47572009000200026.
PMID: 21637694, PMCID: PMC3036925. 27) Araldi RP, de Melo TC, Mendes TB, de Sá Júnior PL, Nozima BH, Ito ET, et al. Using the comet and
micronucleus assays for genotoxicity studies: a review. Biomed Pharmacother. 2015; 72: 74-82. doi:
1016/j.biopha.2015.04.004. PMID: 26054678.
Samanta S, Dey P. Micronucleus and its applications. Diagn Cytopathol. 2012; 40(1): 84-90. doi:
1002/dc.21592. PMID: 22180242.
al-Hawary BA, al-Saleh AA. cytogenetic effects of dacarbazine on mouse bone marrow cells in vivo. Mutat
Res. 1989; 223(2): 259-66. doi: 10.1016/0165-1218(89)90054-2. PMID: 2739683.
Walles SA, Ringborg U. Induction and time course of DNA single-strand breaks in lymphocytes from
patients treated with dacarbazine. Carcinogenesis. 1991; 12(6): 1153-4. doi: 10.1093/carcin/12.6.1153.
PMID: 2044200.
Khan MI, Ahmad I, Mahdi AA, Akhtar MJ, Islam N, Ashquin M, et al. Elevated blood lead levels and
cytogenetic markers in buccal epithelial cells of painters in India: genotoxicity in painters exposed to lead
containing paints. Environ Sci Pollut Res Int. 2010; 17(7): 1347-54. doi: 10.1007/s11356-010-0319-x.
PMID: 20300868.
Adler ID, Kliesch U, Jentsch I, Speicher MR. Induction of chromosomal aberrations by dacarbazine in
somatic and germinal cells of mice. Mutagenesis. 2002; 17(5): 383-9. doi: 10.1007/s11356-010-0319-x.
PMID: 12202625.
Kumar SG, Narayana K, Bairy KL, D'Souza UJ, Samuel VP, Gopalakrishna K. Dacarbazine induces
genotoxic and cytotoxic germ cell damage with concomitant decrease in testosterone and increase in lactate
dehydrogenase concentration in the testis. Mutat Res. 2006; 607(2): 240-52. doi:
1016/j.mrgentox.2006.04.019. PMID: 16793327.
Pourahmad J, Amirmostofian M, Kobarfard F, Shahraki J. Biological reactive intermediates that mediate
dacarbazine cytotoxicity. Cancer Chemother Pharmacol. 2009; 65(1): 89-96. doi: 10.1007/s00280-009- 1007-8. PMID: 19399501.
Quita SM. Evaluation of antioxidant activity of propolis (bee glue) on the histopathology of hepatocytes in
mice treated with dacarbazine. Journal of American Science. 2016; 12(3).
Fu JY, Xia Y, Zheng YY. Antimutagenicity of propolis against some mutagens in vivo and in vetro.
Biomed Environ Sci. 2004; 17(4): 469-75. PMID: 15745252.
Türkez H, Yousef MI. Propolis ameliorates human peripheral blood lymphocytes from DNA damage
caused by aflatoxin B1. Journal of Biological and Environmental Sciences. 2009; 3(9): 77-80.
Aliyazicioglu Y, Demir S, Turan I, Cakiroglu TN, Akalin I, Deger O, et al. Preventive and protective
effects of turkish propolis on H2O2-induced DNA damage in foreskin fibroblast cell lines. Acta Biol Hung.
; 62(4): 388-96. doi: 10.1556/ABiol.62.2011.4.5. PMID: 22119868.
Banskota AH, Nagaoka T, Sumioka LY, Tezuka Y, Awale S, Midorikawa K, et al. Antiproliferative
activity of the Netherlands propolis and its active principles in cancer cell lines. J Ethnopharmacol. 2002;
(1): 67-73. doi: 10.1016/S0378-8741(02)00022-3. PMID: 11891088.
Stan L, Mărghitaş LA, Dezmirean D. Quality criteria for propolis standardization. Scientific Papers Animal
Science and Biotechnologies. 2011; 44(2): 137-40.
Smolarek AK, Suh N. Chemopreventive activity of vitamin E in breast cancer: a focus on γ-and δtocopherol. Nutrients. 2011; 3(11): 962-86. doi: 10.3390/nu3110962. PMID: 22254089, PMCID:
PMC3257724.
Kang TH, Knoff J, Yeh WH, Yang B, Wang C, Kim YS, et al. Treatment of tumors with vitamin E
suppresses myeloid derived suppressor cells and enhances CD8+ T cell-mediated antitumor effects. PloS
one. 2014; 9(7): 103562. doi: 10.1371/journal.pone.0103562. PMID: 25072795, PMCID: PMC4114790.
Khan M, Iqubal A, Joshi A, Ajai K. Role of antioxidants in prevention of cancer: A review. International
Journal of Current Research and Review. 2014; 6(9): 80-8.
Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids.
Fitoterapia. 2011; 82(4): 513-23. doi: 10.1016/j.fitote.2011.01.018.
Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. Scientific World
Journal. 2013; 2013: 162750. doi: 10.1155/2013/162750. PMID: 24470791, PMCID: PMC3891543.
Published
Issue
Section
License
Copyright (c) 2020 KNOWLEDGE KINGDOM PUBLISHING
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.