Evaluation of Antioxidant Activity of R. Slooffiae , R. Mucilaginosa Extracts
Keywords:
Antioxidant, Carotenoid, Rhodotorula, Radical scavenging activityAbstract
Introduction: Antioxidants are health beneficial compounds that can protect cells and macromolecules from the damage of reactive oxygen species (ROS). The aims of this study were to compare the total antioxidant and carotenoid production in R. Slooffiae and R. Mucilaginosa.
Methods: To isolate the carotenoid pigment, cells were suspended in acetone and broken using a homogenizer, followed by centrifugation, and supernatant was separated. For analytical method, pigments were measured spectrophotometrically at 450 nm. The B-carotene bleaching and 1, 1-diphenyl-2-picrylhdrazyl (DPPH) assay were used to determine antioxidant properties of R. Slooffiae and R. Mucilaginosa by measuring the decrease in absorbance at 470 and 517 nm.
Results: The results showed that the content of total carotenoid in R. Slooffiae was higher than R. Mucilaginosa and it presented higher ability to show antioxidant activity. The mean total antioxidant activity of ascorbic acid was the highest (97.11 ± 6.11%), followed by BHT (64.71 ± 5.41%), R. sloofias extract (57.91 ± 7.34%) and R. Mucilaginosa (39.32 ± 5.85%). The EC50 of ascorbic acid was the strongest (0.252 ± 0.000 mg/ml), followed by BHT (0.612 ± 0.009 mg/ml) and R. Slooffiae (0.658 ± 0.033 mg/ml). There was significant difference observed between the EC50 of R. Slooffiae and BHT.
Conclusion: It was found that both strains have ability to produce carotenoid and show antioxidant ability; however, R. Slooffiae had more potential in producing carotenoid and showing antioxidant ability than R. Mucilaginosa. Further study is required, in order to utilize this strain in the food, pharmaceuticals and cosmetics industries.
References
Sies H. Strategies of antioxidant defense. Eur J Biochem. 1993; 215(2): 213-9. doi: 10.1111/j.1432- 1033.1993.tb18025.x. PMID: 7688300.
Hanachi P, Kua SH, Asmah R, Motalleb G, Fauziah O. Cytotoxic effect of Berberis vulgaris fruit extract on
the proliferation of human liver cancer cell line (HepG2) and its antioxidant properties. Int J cancer res.
; 2(1): 1-9. doi: 10.3923/ijcr.2006.1.9.
Young AJ, Lowe GM. Antioxidant and peroxidant properties of carotenoids. Arch Biochem Biophys 2001;
(1): 20-7. doi: 10.1006/abbi.2000.2149. PMID: 11361018.
Krinsky NI. The biological properties of carotenoids. Pure Appl Chem. 1994; 66(5): 1003-10. doi:
1351/pac199466051003.
Naghavi F, Hanachi P, Sabora A. Effect of temperature, PH and salinity on carotenoid production in
Rodotorula Mucilaginosa. Research in Biotechnology. 2014; 5(4): 1-4.
Schroeder WA, Johnson EA. Singlet oxygen and peroxyl radicals regulate carotenoid biosynthesis in
Phaffia rhodozyma. J Biol Chem. 1995; 270(31): 18374-9. doi: 10.1074/jbc.270.31.18374. PMID:
Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA. Antioxidant activities of carotenes and
xanthophylls. FEBS Lett. 1996; 384(3): 240-2. doi: 10.1016/0014-5793(96)00323-7.
Naghavi F, Hanachi P, Sodi MR, Sabora A. The capability of Rhodotorula slooffiae to produce carotenoid.
ZJRMS. 2015; 17(2): 52-6.
Barnett JA, Payne RW, Yarrow D. Yeasts: Characterization and identification, 2nd ed. Cambridge
University Press, Cambridge. 2000; 595-97.
Davies BH. Chemistry and Biochemistry of Plant Pigments. New York, Academic. 1976; 74(1).
Gow-Chin Yen, Chiu-Luan Hsieh. Antioxidant activity of extracts from Du-zhong (Eucommiaulmoides)
toward varioys lipid peroxidation models in vitro. J Agril Food Chem. 1998; 46(10): 3952-57. doi:
1021/jf9800458.
Gazzani G, Papetti A, Massolini G, Daglia M. Anti-and prooxidant activity of water soluble components of
some common diet vegetables and the effect of thermal treatment. J Agril Food Chem. 1998; 46(10): 4118- 22. doi: 10.1021/jf980300o.
Sadat Naghavi F, Hanachi P, Soudi MR, Saboora A, Ghorbani A. Evaluation of the Relationship between
the Incubation Time and Carotenoid Production in Rhodotorula Slooffiae and R. Mucilaginosa Isolated
from Leather Tanning Wastewater. Iran J Basic Med Sci. 2013; 16(10): 1114-18. PMID: 24379970,
PMCID: PMC3874099.
Gunasekera TS, Paul ND, Ayres PG. Responses of phylloplane yeasts to UV-B (290–320 nm) radiation:
interspecific differences in sensitivity. Mycol Res. 1997; 101(7): 779-85. doi:
1017/S0953756296003309.
Moliné M, Libkind D, Diéguez Mdel C, van Broock M. Photoprotective role of carotenoids in yeasts:
Response to UV-B of pigmented and naturally-occurring albino strains. J Photoch Photobio B. 2009;
(3): 156-61. doi: 10.1016/j.jphotobiol.2009.02.006. PMID: 19342251.
Tsimako M, Guffogg S, Thomas-Hall S, Watson K. Resistance to UVB radiation in Antarctic yeasts.
Redox Rep. 2002; 7(5): 312-4. doi: 10.1179/135100002125000866. PMID: 12688517.
Kobayashi M, Kakizono T, Nishio N, Nagai S, Kurimura Y, Tsuji Y. Antioxidant role of astaxanthin in the
green algae Haematococcus pluvialis. Appl Microbiol Biot 1997; 48(3): 351-6. doi:
1007/s002530051061.
Buzzini P, Innocenti M, Turchetti B, Libkind D, van Broock M, Mulinacci N. Carotenoid profiles of yeasts
belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces, and Sporidiobolus. Can J
Microbiol. 2007; 53(8): 1024-31. doi: 10.1139/W07-068. PMID: 17898860.
Sakaki H, Nakanishi T, Satonaka K, Miki W, Fujita T, Komemushi S. Properties of high-torularhodin-pro- ducing mutant of Rhodotrola gulitinis cultivated under oxidative mutant of Rhodotorula glutinis cultivated
under oxidative stress. J Biosci Bioeng. 2000; 89: 203-5. doi: 10.1016/S1389-1723(00)88739-3. PMID:
Chen LS, Ying MA, Maubois JL, Chen LG, Liu QH, Guo JP. Identification of yeasts from raw milk and
selection for some specific antioxidant properties. Society Dairy Technol. 2010; 63(1): 47-54. doi:
1111/j.1471-0307.2009.00548.x.
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